Source was cell extract of COS cell line; bait produced from transfected construct; prey produced as a recombinant fusion protein in bacterial system.

The Mad-tkv interaction is inhibited by cotransfection of Dad.

Cells expressing tkv were incubated with radiolabeled BMP2, then treated with disuccinimdyl suberate cross-linker, which leads to radiolabeling of tkv. Mad was immunoprecipitated and autoradiography was used to detect coimmunoprecipitation of radiolabeled tkv.