Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

Two-hybrid system: yeast GAL4-BD/GAL4-AD

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

The Importin-β-binding domain if Kap-α3 is not necessary for interaction with nkd. nkd did not interact with Kap-α1 or Pen in this experiment.

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Replacement of the D6 domain of nkd with the nuclear localization signal of Hsf duplicates the interaction of wild type nkd with Kap-α3.

Two-hybrid system: yeast GAL4-BD/GAL4-AD