FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
E(spl)m8-HLH, ato
FlyBase ID
FBig0000108358
Interaction Network
Interactions Browser links
E(spl)m8-HLH network
ato network
Reported Interactions
FBrf0216468-3.Y2H
Reference
Eastwood et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
E(spl)m8-HLH 

E(spl)m8-HLH

bait

fused to lexA DNA-binding domain

E(spl)m8-HLH 
ato 

ato

prey

fused to B42 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
E(spl)m8-HLH 
CtD domain
mutation increasing interaction
aa 149-179
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Eastwood et al., 2011)

Two-hybrid system: yeast lexA-BD/B42-AD. Full length E(spl)m8-HLH does not interact with ato. Removal of E(spl)m8-HLH phosphorylation domain allows interaction.

(Eastwood et al., 2011)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)