FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
btl, stumps
FlyBase ID
FBig0000108367
Interaction Network
Interactions Browser links
btl network
stumps network
Reported Interactions
FBrf0160399-55.Y2H
Reference
Battersby et al., 2003
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
stumps 

Dof

bait

fused to GAL4 DNA-binding domain

stumps 
btl 

Breathless

prey

fused to GAL4 activation domain

btl 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
stumps 
N terminal region
sufficient binding region
aa 1-802
btl 
C terminal region
sufficient binding region
aa 795-1052
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Battersby et al., 2003)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Battersby et al., 2003)
FBrf0174960-4.ENZ.A
Reference
Description
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
btl 

Btl

enzyme
btl 

Btl

enzyme
stumps 

Dof

enzyme target

His tag, P[32] label

stumps 

Dof

enzyme target

His tag, P[32] label

stumps 
stumps 
stumps 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
FBrf0174966-1.coIP.WB
Reference
Petit et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
btl 

Btl

bait

MYC tag

stumps 

Dof

prey

V5 tag

stumps 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
stumps 
interaction domain
sufficient binding region
aa 1-484

coordinates relative to stumps-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Petit et al., 2004)

The coIP was performed with an activated form of btl.

(Petit et al., 2004)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)