Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).
Two-hybrid system: yeast GAL4-BD/GAL4-AD, this screen utilized the activation of both a LacZ and His3 reporter gene.
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Two-hybrid system: yeast GAL4-BD/GAL4-AD, this assay utilized an Ade2 reporter gene which the authors claim is more stringent than a LacZ/His3 reporter assay.
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).
Two-hybrid system: yeast GAL4-BD/GAL4-AD
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Two-hybrid system: yeast GAL4-BD/GAL4-AD
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
The coIP was performed with an activated form of htl.
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
