FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
pyd, Cortactin
FlyBase ID
FBig0000108446
Interaction Network
Interactions Browser links
pyd network
Cortactin network
Reported Interactions
FBrf0105255-1.Y2H
Reference
Katsube et al., 1998
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pyd 

ZO-1

bait

fused to GAL4 DNA-binding domain

pyd 
Cortactin 

Cortactin

prey

fused to GAL4 activation domain

Cortactin 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
pyd 
C-terminal region
sufficient binding region
aa 885-1367

coordinates relative to pyd-PB

Cortactin 
C-terminal region
sufficient binding region
aa 464-559

coordinates relative to Cortactin-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

(Katsube et al., 1998)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Katsube et al., 1998)
FBrf0105255-2.Y2H
Reference
Katsube et al., 1998
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pyd 

ZO-1

bait

fused to GAL4 DNA-binding domain

pyd 
Cortactin 

Cortactin

prey

fused to GAL4 activation domain

Cortactin 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Cortactin 
C-terminal region
sufficient binding region
aa 464-559

coordinates relative to Cortactin-PA

pyd 
Proline-rich domain
sufficient binding region
aa 1115-1253

coordinates relative to pyd-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Katsube et al., 1998)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Katsube et al., 1998)
FBrf0105255-3.coIP.WB
Reference
Katsube et al., 1998
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cortactin 

Cortactin

bait
pyd 

ZO-1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Katsube et al., 1998)
FBrf0105255-4.coIP.WB
Reference
Katsube et al., 1998
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pyd 

ZO-1

bait
Cortactin 

Cortactin

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Katsube et al., 1998)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)