FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
dsx
FlyBase ID
FBig0000108461
Interaction Network
Interactions Browser links
dsx network
Reported Interactions
FBrf0091055-1.Y2H
Reference
Erdman et al., 1996
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dsx 

dsx

bait

fused to lexA DNA-binding domain

dsx 

dsx

prey

fused to B42 activation domain

dsx 
dsx 
dsx 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
dsx 
N-terminal region
sufficient binding region
aa 1-159
dsx 
C-terminal region
sufficient binding region
aa 298-427

coordinates relative to dsx-PB isoform

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Erdman et al., 1996)
FBrf0091055-2.XL.WB
Reference
Erdman et al., 1996
Description
physical association
Assay
Collection
Source/Stage
organism / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dsx 

dsx

bait

FLAG tag

dsx 

dsx

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adults of transgenic fly line; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.

(Erdman et al., 1996)

Cross-linking of proteins was

(Erdman et al., 1996)

accomplished by the addition of 0.01% glutaraldehyde to protein extracts.

(Erdman et al., 1996)
FBrf0204143-1.NMR
Reference
Yang et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dsx 

DSX

neutral component

female-specific isoform

dsx 

DSX

neutral component

female-specific isoform

dsx 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
dsx 
CTD domain
sufficient binding region
aa 350-427

coordinates relative to dsx-PC

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; protein produced as a recombinant fusion proteins in bacterial system.

(Yang et al., 2008)
FBrf0204143-4.Y2H
Reference
Yang et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dsx 

DSX

bait

fused to GAL4 DNA-binding domain

dsx 

DSX

prey

fused to GAL4 activation domain

dsx 
dsx 
dsx 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
dsx 
interaction domain
sufficient binding region
aa 350-427

coordinates relative to dsx-PC

dsx 
interaction domain
mutation disrupting interaction
aa 394aa 379

substituting R394 with K has no effect on dimerization, substituting with A,G,Q,N,E,D,C,T,M or P disrupts dimerization

substituting V379 with A,T,G,L,F,E or R disrupts dimerization

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Yang et al., 2008)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Yang et al., 2008)
External Crossreferences and Linkouts ( 0 )
References (2)