FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Eb1, ncd
FlyBase ID
FBig0000108479
Interaction Network
Interactions Browser links
Eb1 network
ncd network
Reported Interactions
FBrf0180068-4.PD.MS
Reference
Rogers et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Eb1 

EB1

bait

GST tag

ncd 

Ncd

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from S2 cell extract.

(Rogers et al., 2004)
FBrf0184181-2.PD.WB
Reference
Mennella et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Eb1 

Eb1

bait

GST tag

ncd 

ncd

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from S2 cell extract

(Mennella et al., 2005)
FBrf0234754-1.FRET.FM
Reference
Szczęsna and Kasprzak, 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ncd 

Ncd

fluorescence donor

GFP tag

ncd 

Ncd

fluorescence donor

GFP tag

ncd 
ncd 

Ncd

fluorescence donor

GFP tag

ncd 
Eb1 

EB1

fluorescence acceptor

QSY35 label

Eb1 

EB1

fluorescence acceptor

QSY35 label

Eb1 

EB1

fluorescence acceptor

QSY35 label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ncd 
tail
sufficient binding region
aa 1-349

coordinates relative to ncd-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; fluorescence donor produced from tagged transgenic construct; fluorescence acceptor produced from tagged transgenic construct.

(Szczęsna and Kasprzak, 2016)

Kd = 8.6 uM.

(Szczęsna and Kasprzak, 2016)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)