FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000108483
Interaction Network
Interactions Browser links
mir-276a network
tim network
Reported Interactions
FBrf0232489-1.miR-mRNA.LUC
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

mir-276a

bait
tim 

tim

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Overexpression of mir-276a decreases tim 3-prime UTR reporter activity.

Source was S2 cell line; bait produced from transfected construct; prey produced from transfected reporter construct.

Luciferase reporter assay.

FBrf0232489-2.miR-mRNA.LUC
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

mir-276a

bait
tim 

tim

prey
tim 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
tim 
3-prime UTR
mutation decreasing interaction
AGUUCCU

GUUCC to CAAGG

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was S2 cell line; bait produced from endogenous gene; prey produced from transfected reporter construct.

Mutation of mir-276a binding site in tim 3-prime UTR increases tim 3-prime UTR reporter activity.

Luciferase reporter assay.

FBrf0232489-3.miR-mRNA.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

mir-276a

bait
tim 

tim

prey
tim 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
tim 
3-prime UTR
mutation decreasing interaction
AGUUCCU
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of wild-type or mutant fly line; bait produced from endogenous gene; prey produced from endogenous gene.

Mutation of mir-276a binding site in tim 3-prime UTR leads to increase in tim protein.

FBrf0247672-1.miR-mRNA.LUC
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

miR-276a

bait
tim 

tim

prey
tim 
tim 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
tim 
3-prime UTR
sufficient binding region
tim 
mir-276a target site
necessary binding region
AGTTCCT
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Luciferase reporter assay.

Source was S2 cell line; bait produced from transfected construct; prey produced from transfected reporter construct.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)