FB2026_03 , released September 17, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
nanos, Cnot4
FlyBase ID
FBig0000108484
Interaction Network
Interactions Browser links
nanos network
Cnot4 network
Reported Interactions
FBrf0194692-7.Y2H
Reference
Kadyrova et al., 2007
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
nanos 

Nos

bait

fused to GAL4 DNA-binding domain

nanos 
Cnot4 

NOT4

prey

fused to GAL4 activation domain

Cnot4 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Cnot4 
C-terminal region
sufficient binding region
aa 597-1051

coordinates relative to Cnot4-PA

nanos 
N-terminal region
sufficient binding region
aa 1-288

coordinates relative to nos-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Kadyrova et al., 2007)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Kadyrova et al., 2007)
FBrf0260793-3.Y2H
Reference
Marhabaie et al., 2024
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
nanos 

nanos

bait

fused to GAL4 DNA-binding domain

nanos 
Cnot4 

NOT4

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
nanos 
N-terminal domain
sufficient binding region
aa 1-289
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Marhabaie et al., 2024)

Two-hybrid system: yeast GAL4-BD/GAL4-AD; positive control

(Marhabaie et al., 2024)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)