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FB2026_01
,
released March 12, 2026
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from adult cDNA expression library).
Two-hybrid system: yeast GAL4-BD/GAL4-AD
Interaction in vitro; bait produced as a recombinant fusion protein in 293T cells; prey derived from accessory gland extract.
Interaction in vitro; bait produced as a recombinant fusion protein in 293T cells; prey derived from reproductive system extract.
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Two-hybrid system: yeast GAL4-BD/GAL4-AD
Source was male accessory glands of wild-type adults; protein produced from endogenous gene.
Source was reproductive tracts of mated wild-type females; protein produced from endogenous gene.
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Source was male accessory glands of wild-type fly line; proteins produced from endogenous genes.
L229A,L232A
S236A,I239A
I243A,V246A
Y224A,Y228A
Interaction in vitro; proteins produced tagged in cell free bacterial system.