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FB2026_01
,
released March 12, 2026
ovulin, Mst26Aa, Acp26A, Acp26Aab, Mst26A
male ejaculate accessory gland protein - increases ovulation through octopamine based neuronal signaling
Please see the JBrowse view of Dmel\Acp26Aa for information on other features
To submit a correction to a gene model please use the Contact FlyBase form
AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.
Gene model reviewed during 5.51
0.9 (northern blot)
There is only one protein coding transcript and one polypeptide associated with this gene
41, 37, 33, 30, 28, 25 (kD observed)
29, 22 (kD observed)
264 (aa); 41, 37, 36 (kD observed); 29 (kD predicted)
The 41kD, 37kD and 36kD forms of Acp26Aa protein differ in the extent of glycosylation. The 36kD species is far less abundant than the other two. By tracking with region-specific antibodies, it was shown that the 41kD species is processed to 38kD, 33kD and finally to 28kD while the 37kD species is processed to 33kD, 30kD and finally 25kD in parallel processing pathways. These processing steps were shown to consist of stepwise cleavage events in the N-terminal half of the protein. The processing sites were mapped by CNBr mapping and by analysis of conserved cleav
ge sites within several Drosophila species. Finally, it was found that accessory gland main cell secretions are necessary to process Acp26Aa protein in the female genital tract.
This putative cleavage product was not detected. It is thought to be generated from the 41kD product by an N-terminal cleavage step in the female genital tract.
The 41kD, 37kD and 36kD forms of Acp26Aa protein differ in the extent of glycosylation. The 36kD species is far less abundant than the other two. By tracking with region-specific antibodies, it was shown that the 41kD species is processed to 38kD, 33kD and finally to 28kD while the 37kD species is processed to 33kD, 30kD and finally 25kD in parallel processing pathways. These processing steps were shown to consist of stepwise cleavage events in the N-terminal half of the protein. The processing sites were mapped by CNBr mapping and by analysis of conservated cle
vage sites within several Drosophila species. Finally, it was found that accessory gland main cell secretions are necessary to process Acp26Aa protein in the female genital tract.
Thought to be generated from a putative 38kD product by an N-terminal cleavage step in the female genital tract.
Generated from the 37kD species by an N-terminal cleavage step in the female genital tract.
Three forms of Acp26Aa protein are present in the male accessory gland, 36, 37, and 41kD. The 37kD is the most abundant. This is cleaved to 30kD in the female genital tract by the end of mating. There may be a 33kD intermediate.
Generated from a 33kD species by an N-terminal cleavage step in the female genital tract.
Generated from the 30kD species by an N-terminal cleavage step in the female genital tract.
Homodimer.
Glycosylation.
Undergoes several cleavages as it is secreted and is further processed in the recipient female (PubMed:10612039, PubMed:2257979, PubMed:24514904, PubMed:3142802, PubMed:7556947). The precursor molecule is proteolytically cleaved by the seminal metalloprotease Semp1 at Lys-48 to produce CP1-N and CP1-C (PubMed:2257979, PubMed:24514904, PubMed:3142802, PubMed:7556947).
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Acp26Aa using the Feature Mapper tool.
The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).
Acp26Aa transcripts were assayed in hand-sorted staged pupae. They are first detected at pupal stage 13. They are present at adult levels by one day after eclosion and persist at these levels for at least 10 days. Determination of expression occurs during the third larval instar which correlates with the time of determination of the accessory glands.
Acp26Aa transcripts are detected in RNA from adult males and specifically in accessory gland RNA.
Comment: mated females
Comment: mated females
Comment: mated females
Comment: mated females
The processed forms of Acp26Aa protein are detected in the female genital tract.
The 30kD processed form of Acp26Aa protein is the major form in the female genital tract by the end of mating.
The 25kD processed form of Acp26Aa protein is the major form in the female genital tract 30 minutes after mating, and by 2 hours, it is the only form detected.
JBrowse - Visual display of RNA-Seq signals
View Dmel\Acp26Aa in JBrowse2-18
2-19.0
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
polyclonal
If females remate frequently relative to the duration of sperm storage and rate of sperm use, sperm displacement may be an important component of male reproductive success. Significant association has been found between Acp26Aa mutant male sperm and the ability to resist displacement by subsequent sperm. There is no correlation between the ability to displace resident sperm and the ability to resist being displaced by subsequent sperm, this lack of correlation suggests that different mechanisms mediate that the two components of sperm displacement.
Acp26Aa contributes to the initial (first day postmating) stimulation of egg laying in the mated female.
The Acp26Aa product is transferred to the female from the male during mating, and is processed in the female genital tract. Processing involves sequential proteolytic cleavage which is dependent on activities donated by the male.
Sequence and restriction map polymorphism have been studied in 10 isogenic lines of D.melanogaster, and one line each of D.sechellia, D.mauritiana and D.simulans : pattern suggests selection in or near Acp26Aa region has played a part in the history of these genes.
The developmental and mating-stimulated expression of Acp26Aa, and the transfer of the protein product to and fates in the mated female has been studied.
