FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
14-3-3ε, Rip11
FlyBase ID
FBig0000108610
Interaction Network
Interactions Browser links
14-3-3ε network
Rip11 network
Reported Interactions
FBrf0234562-1.PD.MS
Reference
Laflamme et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
14-3-3ε 

14-3-3ε

bait

GST tag

Rip11 

Rip11

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from S2 cell extract.

(Laflamme et al., 2017)

Rip11 was most abundant protein identified bound to GST-14-3-3ε column.

(Laflamme et al., 2017)
FBrf0234562-2.PD.WB
Reference
Laflamme et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
14-3-3ε 

14-3-3ε

bait

GST tag

Rip11 

Rip11

prey

GFP tag

Rip11 
Rip11 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Rip11 
interaction domain
necessary binding region
aa 352-409

coordinates relative to Rip11-PB

Rip11 
interaction domain
mutation disrupting interaction
aa 391,405

\'TS/AA\' is T391A, S405A, coordinates relative to Rip11-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced from tagged transfected construct.

(Laflamme et al., 2017)
FBrf0234562-3.FW.WB
Reference
Laflamme et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Rip11 

Rip11

bait

GFP tag

14-3-3ε 

14-3-3ε

prey

GST tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced from tagged transfected construct; prey produced as a recombinant fusion protein in bacterial system.

(Laflamme et al., 2017)
FBrf0234562-5.coIP.WB
Reference
Laflamme et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
14-3-3ε 

14-3-3ε

bait

myc tag

Rip11 

Rip11

prey

GFP tag

Rip11 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Rip11 
interaction domain
mutation disrupting interaction
aa 391,405

\'TS/AA\' is T391A, S405A, coordinates relative to Rip11-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from tagged transfected construct; prey produced from tagged transfected construct.

(Laflamme et al., 2017)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)