Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: DNA binding domain and activation domain unspecified.

Source was S2 cell line; bait produced from tagged transfected construct; prey produced from tagged transfected construct.

Bait protein localizes to cell cortex while prey is largely cytoplasmic. The ability of this bait to recruit prey protein to cell cortex when co-expressed was taken as evidence of direct physical interaction.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as synthetic peptides.

pyd Ex6 peptide binds to WW2, WW1, WW1-2, WW3-4 domains of Su(dx) with decreasing affinity. pyd Pro-rich peptide binds only WW1-2 domain of Su(dx).