FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
pum, eIF4E1
FlyBase ID
FBig0000108695
Interaction Network
Interactions Browser links
pum network
eIF4E1 network
Reported Interactions
FBrf0180581-2.EMSA.A
Reference
Menon et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pum 

Pum

bait

GST tag

pum 
eIF4E1 

eIF4E

prey

[32]P label

eIF4E1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
pum 
RBD
sufficient binding region
aa 1091-1433

coordinates relative to pum-PA

eIF4E1 
eIF-4E 3UTR binding sequence
sufficient binding region
GCAUAAUAUAAAAUCUAUCCGCUUUUUGUAGUCACUGUCAAUAAUGGAUUA
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion proteins in bacterial system; prey produced and labeled in vitro.

(Menon et al., 2004)

Positive control.

(Menon et al., 2004)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)