FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
sqd, Imp
FlyBase ID
FBig0000108734
Interaction Network
Interactions Browser links
sqd network
Imp network
Reported Interactions
FBrf0194192-1.coIP.WB
Reference
Geng and Macdonald, 2006
Description
physical association
Assay
Collection
Source/Stage
ovary / adult stagefemale
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Imp 

Imp

bait
sqd 

Sqd

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult female ovaries of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Geng and Macdonald, 2006)

sqd coimmunoprecipitates with Imp in an RNA-dependent way (interaction disrupted by RNaseA/T1).

(Geng and Macdonald, 2006)
FBrf0203066-3.coIP.MS
Reference
Clouse et al., 2008
Description
physical association
Assay
Collection
Source/Stage
ovary / adult stagefemale
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sqd 

Sqd

bait
Imp 

Imp

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult female ovaries of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Clouse et al., 2008)

Copurified proteins were identified only in sqd immunoprecipitation, and not in the negative control (dl immunoprecipitation).

(Clouse et al., 2008)
FBrf0228789-1.EMSA.A
Reference
Hansen et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Imp 

Imp

bait

His tag

sqd 

sqd

prey

[32]P label

sqd 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
sqd 
3-prime UTR
sufficient binding region
unspecified
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro transcription.

(Hansen et al., 2015)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)