Source was S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
N terminal tagged sav showed the strongest interaction in this experiment when compared with C terminally tagged or combinations of N and C terminally tagged proteins.
Source was cell extract of HEK293T cell line; bait produced from transfected construct; prey produced from transfected construct.
sav forms a homodimer in solution.
Interaction in vitro; protein produced as a recombinant fusion protein in bacterial system.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.
