FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
RhoGEF2, T48
FlyBase ID
FBig0000108863
Interaction Network
Interactions Browser links
RhoGEF2 network
T48 network
Reported Interactions
FBrf0192307-3.PD.A
Reference
Kolsch et al., 2007
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
RhoGEF2 

RhoGEF2

bait

GST tag

RhoGEF2 
T48 

T48

prey

[35]S label

T48 
T48 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
RhoGEF2 
PDZ domain
sufficient binding region

coordinates not reported

T48 
intracellular domain
sufficient binding region
aa 183-419

coordinates relative to T48-PA

T48 
C-terminal ITTEL motif
necessary binding region
DEITTEL
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Kolsch et al., 2007)
FBrf0216703-4.PD.A
Reference
Mathew et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
RhoGEF2 

RhoGEF2

bait

GST tag

RhoGEF2 
T48 

T48intra

prey

[35]S label

T48 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
RhoGEF2 
PDZ domain
sufficient binding region

coordinates not reported

T48 
intracellular domain
sufficient binding region
aa 183-419

coordinates relative to T48-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Mathew et al., 2011)

Positive control.

(Mathew et al., 2011)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)