Mutations that disrupt Hsc70-4 chaperone function (K3A, D10N) did not affect its interaction with Sgt. Sgt inhibits the membrane deformation activity of Hsc70-4.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

Hsc70-4 binds Sgt but not Lamp1.

Source was adult heads of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.