FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
cno, pyd
FlyBase ID
FBig0000108883
Interaction Network
Interactions Browser links
cno network
pyd network
Reported Interactions
FBrf0105943-1.Y2H
Reference
Takahashi et al., 1998
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
cno 

cno

bait

fused to GAL4 DNA-binding domain

cno 
cno 
pyd 

ZO-1

prey

fused to GAL4 activation domain

pyd 
pyd 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
cno 
N-terminal region
sufficient binding region
aa 1-950

coordinates relative to cno-PC

cno 
C-terminal region
sufficient binding region
aa 800-1882

coordinates relative to cno-PC

pyd 
N-terminal region
sufficient binding region
aa 1-284

"T1" construct, coordinates relative to pyd-PB

pyd 
C-terminal region
sufficient binding region
aa 885-1367

"T4" construct, coordinates relative to pyd-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Takahashi et al., 1998)

Binding is mediated by two different regions. The N-terminal regions of cno and pyd bind each other, as do their C-terminal regions.

(Takahashi et al., 1998)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Takahashi et al., 1998)
FBrf0105943-2.PD.A
Reference
Takahashi et al., 1998
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pyd 

ZO-1

bait

GST tag

pyd 
pyd 
cno 

Cno

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
pyd 
N-terminal region
sufficient binding region
aa 1-284

"T1" construct, coordinates relative to pyd-PB

pyd 
C-terminal region
sufficient binding region
aa 1098-1260

"T11" construct, coordinates relative to pyd-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Takahashi et al., 1998)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)