FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000109041
Interaction Network
Interactions Browser links
His4 network
Parp1 network
Reported Interactions
FBrf0224797-9.PD.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
His4 

His4

bait

linked to beads

His4 
His4 
His4 

Parp

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
His4 
C-terminus
mutation decreasing interaction
unspecified

fragment consisting of N-terminal tail binds weakly

His4 
C-terminus
sufficient binding region
unspecified

fragment with N-terminal tail deleted still binds.

His4 
residue 61
mutation disrupting interaction
aa 61

V61G

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

FBrf0242383-1.coIP.WB
Description
physical association
Collection
Cell line used
Participants
Corresponds to
Reported as
Role
Note

PARP-1

bait

YFP tag

His4 

H4

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
W-Cat domain
sufficient binding region
aa 387-994

coordinates relative to Parp-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larval salivary glands of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

FBrf0242383-3.PD.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
His4 

H4

bait
His4 

PARP-1

prey

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
W-Cat domain
sufficient binding region
aa 387-994

coordinates relative to Parp-PB

His4 
interaction domain residues 23,61
mutation disrupting interaction
aa 23,61

L23G,V61G

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

External Crossreferences and Linkouts ( 0 )
References (2)