FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
sav, Herc4
FlyBase ID
FBig0000109155
Interaction Network
Interactions Browser links
sav network
Herc4 network
Reported Interactions
FBrf0231779-2.coIP.WB
Reference
Aerne et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
sav 

sav

bait

Myc tag

sav 
Herc4 

Sherpa

prey

FLAG tag

Herc4 
Herc4 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
sav 
interaction domain
sufficient binding region
aa 294-608

coordinates relative to sav-PA

Herc4 
interaction domain
sufficient binding region
aa 158-1059aa 1-710

coordinates relative to Sherpa-PA

coordinates relative to Sherpa-PA

Herc4 
RCC1 repeat domain
necessary binding region
aa 1-400

coordinates relative to Sherpa-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Aerne et al., 2015)
FBrf0231779-3.coIP.WB
Reference
Aerne et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Herc4 

Sherpa

bait

FLAG tag

sav 

sav

prey

Myc tag

sav 
sav 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
sav 
interaction domain
sufficient binding region
aa 420-546aa 492-608

coordinates relative to sav-PA

coordinates relative to sav-PA

sav 
interaction domain
necessary binding region
aa 492-608

coordinates relative to sav-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Aerne et al., 2015)

Addition of hpo competes/decreases interaction.

(Aerne et al., 2015)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)