FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
ena, Khc
FlyBase ID
FBig0000109227
Interaction Network
Interactions Browser links
ena network
Khc network
Reported Interactions
FBrf0188046-1.Y2H
Reference
Martin et al., 2005
Description
physical association
Assay
Collection
Source/Stage
organism / larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ena 

Ena

bait

fused to GAL4 DNA-binding domain

ena 
Khc 

Khc

prey

fused to GAL4 activation domain

Khc 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ena 
Ena-VASP homology domains domain
sufficient binding region
aa 441-684

coordinates relative to ena-PA

Khc 
C-terminal domain
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from larval cDNA expression library).

(Martin et al., 2005)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Martin et al., 2005)
FBrf0188046-2.coIP.WB
Reference
Martin et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
ena 

Ena

bait
Khc 

Khc

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Martin et al., 2005)
FBrf0188046-3.coIP.WB
Reference
Martin et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Khc 

Khc

bait
ena 

Ena

prey
ena 
ena 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ena 
ena lacking the 52 C-terminal amino acids
sufficient binding region
aa 1-632

coordinates relative to ena-PA

ena 
C-terminal domain
necessary binding region
aa 441-632

coordinates relative to ena-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Martin et al., 2005)
FBrf0188046-4.coIP.WB
Reference
Martin et al., 2005
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ena 

Ena

bait
Khc 

Khc

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Martin et al., 2005)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)