FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
tll, Gug
FlyBase ID
FBig0000109370
Interaction Network
Interactions Browser links
tll network
Gug network
Reported Interactions
FBrf0190568-2.Y2H
Reference
Wang et al., 2006
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
tll 

Tll

bait

fused to GAL4 DNA-binding domain

Gug 

Atro

prey

fused to GAL4 activation domain

Gug 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Gug 
interaction domain
sufficient binding region
aa 1301-1966

coordinates relative to Gug-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Wang et al., 2006)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

(Wang et al., 2006)
FBrf0190568-3.Y2H
Reference
Wang et al., 2006
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
tll 

Tll

bait

fused to GAL4 DNA-binding domain

tll 
tll 
Gug 

Atro

prey

fused to GAL4 activation domain

Gug 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Gug 
interaction domain
sufficient binding region
aa 1711-1966

coordinates relative to Gug-PA

tll 
ligand-binding domain
sufficient binding region
aa 192-452

coordinates relative to tll-PA

tll 
ligand-binding domain
necessary binding region
aa 353-452

coordinates relative to tll-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Wang et al., 2006)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Wang et al., 2006)
FBrf0190568-4.PD.A
Reference
Wang et al., 2006
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Gug 

Atro

bait

GST tag

Gug 
tll 

Tll

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Gug 
interaction domain
sufficient binding region
aa 1711-1966

coordinates relative to Gug-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Wang et al., 2006)
FBrf0201761-4.PD.A
Reference
Haecker et al., 2007
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
tll 

Tll

bait

GST tag

tll 
Gug 

Atro

prey

[35]S label

Gug 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
tll 
protein lacking the DNA binding domain
sufficient binding region
aa 101-542
Gug 
C-terminal domain
sufficient binding region
aa 1324-1966
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Haecker et al., 2007)
FBrf0201761-6.coIP.WB
Reference
Haecker et al., 2007
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Gug 

Atro

bait
tll 

Tll

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from transfected construct.

(Haecker et al., 2007)
FBrf0224065-1.BiFC.FM
Reference
Davis et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Gug 

Gug

neutral component

YFP tag, N-terminal fragment

Gug 

Gug

neutral component

YFP tag, N-terminal fragment

Gug 
Gug 

Gug

neutral component

YFP tag, N-terminal fragment

Gug 
tll 

tll

neutral component

YFP tag, C-terminal fragment

tll 

tll

neutral component

YFP tag, C-terminal fragment

tll 

tll

neutral component

YFP tag, C-terminal fragment

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Gug 
C-terminal region
sufficient binding region
unspecified
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was live S2 cells; proteins produced from transfected constructs.

(Davis et al., 2014)
FBrf0224065-2.BiFC.FM
Reference
Davis et al., 2014
Description
physical association
Assay
Collection
Source/Stage
brain / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Gug 

Gug

neutral component

YFP tag, N-terminal fragment

Gug 

Gug

neutral component

YFP tag, N-terminal fragment

Gug 
Gug 

Gug

neutral component

YFP tag, N-terminal fragment

Gug 
tll 

tll

neutral component

YFP tag, C-terminal fragment

tll 

tll

neutral component

YFP tag, C-terminal fragment

tll 

tll

neutral component

YFP tag, C-terminal fragment

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Gug 
C-terminal region
sufficient binding region
unspecified
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult brain of transgenic fly line; proteins produced from tagged transgenic constructs.

(Davis et al., 2014)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)