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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000109419
Interaction Network
Interactions Browser links
Ubx network
al network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0226424-1.Y2H
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Ubx 

Ubx

bait

fused to lexA DNA-binding domain

Ubx 
Ubx 
Ubx 
Ubx 
Ubx 
al 

al

prey

fused to B42 activation domain

al 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
al 
interaction domain
sufficient binding region
aa 81-142
Ubx 
interaction domain
sufficient binding region
aa 1-291

coordinates refer to Ubx-PA

Ubx 
interaction domain
necessary binding region
aa 1-215

coordinates refer to Ubx-PA

Ubx 
interaction domain
mutation decreasing interaction
aa 1-103aa 103-216aa 250-292

coordinates refer to Ubx-PA

coordinates refer to Ubx-PA

coordinates refer to Ubx-PA

Ubx 
interaction domain
mutation disrupting interaction
aa 1-103,250-292aa 1-103,292-389aa 103-216,250-292

coordinates refer to Ubx-PA

coordinates refer to Ubx-PA

coordinates refer to Ubx-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Transcriptionally inactive mutant A226P,Q233P of Ubx used.

FBrf0240351-168.BiFC.FM
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
al 

al

bait

YFP tag, N-terminal fragment

Ubx 

Ubx

prey

YFP tag, C-terminal fragment

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; proteins produced from tagged transgenic constructs.

FBrf0240351-332.BiFC.FM
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Ubx 

Ubx

bait

YFP tag, N-terminal fragment

al 

al

prey

CFP tag, C-terminal fragment

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; proteins produced from tagged transgenic constructs.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)