FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Ubx, al
FlyBase ID
FBig0000109419
Interaction Network
Interactions Browser links
Ubx network
al network
Reported Interactions
FBrf0226424-1.Y2H
Reference
Hsiao et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Ubx 

Ubx

bait

fused to lexA DNA-binding domain

Ubx 
Ubx 
Ubx 
Ubx 
Ubx 
al 

al

prey

fused to B42 activation domain

al 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
al 
interaction domain
sufficient binding region
aa 81-142
Ubx 
interaction domain
sufficient binding region
aa 1-291

coordinates refer to Ubx-PA

Ubx 
interaction domain
necessary binding region
aa 1-215

coordinates refer to Ubx-PA

Ubx 
interaction domain
mutation decreasing interaction
aa 1-103aa 103-216aa 250-292

coordinates refer to Ubx-PA

coordinates refer to Ubx-PA

coordinates refer to Ubx-PA

Ubx 
interaction domain
mutation disrupting interaction
aa 1-103,250-292aa 1-103,292-389aa 103-216,250-292

coordinates refer to Ubx-PA

coordinates refer to Ubx-PA

coordinates refer to Ubx-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Hsiao et al., 2014)

Transcriptionally inactive mutant A226P,Q233P of Ubx used.

(Hsiao et al., 2014)
FBrf0240351-168.BiFC.FM
Reference
Bischof et al., 2018
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
al 

al

bait

YFP tag, N-terminal fragment

Ubx 

Ubx

prey

YFP tag, C-terminal fragment

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; proteins produced from tagged transgenic constructs.

(Bischof et al., 2018)
FBrf0240351-332.BiFC.FM
Reference
Bischof et al., 2018
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Ubx 

Ubx

bait

YFP tag, N-terminal fragment

al 

al

prey

CFP tag, C-terminal fragment

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; proteins produced from tagged transgenic constructs.

(Bischof et al., 2018)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)