FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Gfrl, Ret
FlyBase ID
FBig0000109420
Interaction Network
Interactions Browser links
Gfrl network
Ret network
Reported Interactions
FBrf0235466-1.PD.WB
Reference
Saarenpää et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Gfrl 

Gfrl

bait

His tag

Ret 

Ret

prey

FLAG tag

Ret 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Ret 
interaction domain
sufficient binding region
aa 30-520
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Gfrl isoform A used.

(Saarenpää et al., 2017)

Interaction in vitro; bait produced as a recombinant fusion protein in baculovirus and insect cell system; prey produced as a recombinant fusion protein in baculovirus and insect cell system.

(Saarenpää et al., 2017)
FBrf0235466-2.PD.MW
Reference
Saarenpää et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Gfrl 

Gfrl

bait

His tag

Ret 

Ret

prey
Ret 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Ret 
extra-cellular domain
sufficient binding region
aa 30-696
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Gfrl isoform Ab used.

(Saarenpää et al., 2017)

Interaction in vitro; bait produced as a recombinant fusion protein in baculovirus and insect cell system; prey produced as a recombinant fusion protein in baculovirus and insect cell system.

(Saarenpää et al., 2017)
FBrf0238088-2.coIP.WB
Reference
Myers et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Gfrl 

Gfrl

bait

V5 tag

Ret 

Ret

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of HEK 293 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Myers et al., 2018)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)