sturkopf, Gp93 Physical Interaction report

General Information

Interaction Type

physical association

Interacting Genes

sturkopf, Gp93

FlyBase ID

FBig0000109468

Interaction Network

Interactions Browser links

sturkopf network
Gp93 network

Reported Interactions

FBrf0234959-24.BiLC.LUC

Reference

Kolkhof et al., 2017

Description

physical association

Assay

split luciferase complementation

luminiscence technology

Collection

Source/Stage

Cell line used

Kc167

Participants

Corresponds to

Reported as

Role

Note

sturkopf

CG9186

neutral component

Gaussia luciferase tag, N-terminal fragment

sturkopf

CG9186

neutral component

Gaussia luciferase tag, N-terminal fragment

sturkopf

CG9186

neutral component

Gaussia luciferase tag, N-terminal fragment

Gp93

neutral component

Gaussia luciferase tag, C-terminal fragment

Gp93

neutral component

Gaussia luciferase tag, C-terminal fragment

Gp93

neutral component

Gaussia luciferase tag, C-terminal fragment

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Weak interaction.

(Kolkhof et al., 2017)

Source was Kc167 cells; proteins produced from transfected constructs.

(Kolkhof et al., 2017)

Cells treated with 400uM oleic acid to induce lipid droplet formation in cells.

(Kolkhof et al., 2017)

FBrf0234959-29.BiLC.LUC

Reference

Kolkhof et al., 2017

Description

physical association

Assay

split luciferase complementation

luminiscence technology

Collection

Source/Stage

Cell line used

Kc167

Participants

Corresponds to

Reported as

Role

Note

Gp93

neutral component

Gaussia luciferase tag, N-terminal fragment

Gp93

neutral component

Gaussia luciferase tag, N-terminal fragment

Gp93

neutral component

Gaussia luciferase tag, N-terminal fragment

sturkopf

CG9186

neutral component

Gaussia luciferase tag, C-terminal fragment

sturkopf

CG9186

neutral component

Gaussia luciferase tag, C-terminal fragment

sturkopf

CG9186

neutral component

Gaussia luciferase tag, C-terminal fragment

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Strong interaction.

(Kolkhof et al., 2017)

Source was Kc167 cells; proteins produced from transfected constructs.

(Kolkhof et al., 2017)

Cells treated with 400uM oleic acid to induce lipid droplet formation in cells.

(Kolkhof et al., 2017)

FBrf0234959-69.coIP.MS

Reference

Kolkhof et al., 2017

Description

physical association

Assay

anti tag coimmunoprecipitation

Identification by mass spectrometry

Collection

Source/Stage

Cell line used

Kc167

Participants

Corresponds to

Reported as

Role

Note

sturkopf

CG9186

bait

GFP tag

Gp93

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of Kc167 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Kolkhof et al., 2017)

Cells treated with 400uM oleic acid to induce lipid droplet formation in cells.

(Kolkhof et al., 2017)

External Crossreferences and Linkouts ( 1 )

Linkouts

MIST - An integrated Molecular Interaction Database

CG9186,Gp93

References (1)

Report Sections

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