FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Klp64D, ChAT
FlyBase ID
FBig0000109475
Interaction Network
Interactions Browser links
Klp64D network
ChAT network
Reported Interactions
FBrf0191019-5.coIP.WB
Reference
Baqri et al., 2006
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ChAT 

ChAT

bait
Klp64D 

KLP64D

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Baqri et al., 2006)

Syx1A failed to bind Klp64D in this experiment.

(Baqri et al., 2006)
FBrf0218568-2.PD.WB
Reference
Sadananda et al., 2012
Description
physical association
Assay
Collection
Source/Stage
head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Klp64D 

KLP64D

bait

His tag

Klp64D 
Klp64D 
ChAT 

ChAT

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Klp64D 
tail fragment
sufficient binding region
aa 590-677
Klp64D 
tail fragment
necessary binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from adult heads of transgenic fly line.

(Sadananda et al., 2012)

Klp68D did not bind to ChAT in this experiment.

(Sadananda et al., 2012)
FBrf0218568-3.PD.WB
Reference
Sadananda et al., 2012
Description
physical association
Assay
Collection
Source/Stage
head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Klp64D 

KLP64D

bait

GST tag

Klp64D 
ChAT 

ChAT

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Klp64D 
tail fragment
sufficient binding region
aa 590-677
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from adult heads of transgenic fly line.

(Sadananda et al., 2012)

Klp68D did not bind to ChAT in this experiment.

(Sadananda et al., 2012)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)