FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Hsp83, Met
FlyBase ID
FBig0000109526
Interaction Network
Interactions Browser links
Hsp83 network
Met network
Reported Interactions
FBrf0226403-1.coIP.WB
Reference
He et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Hsp83 

Hsp83

bait

Myc tag

Hsp83 
Hsp83 
Met 

Met

prey

V5 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Hsp83 
bHLH domain
necessary binding region
aa 70-76
Hsp83 
PAS B domain
necessary binding region
aa 428-438
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of Kc cell line; bait produced from transfected construct; prey produced from transfected construct.

(He et al., 2014)

Treatment of cells with methoprene, a juvenile hormone analog, increases interaction.

(He et al., 2014)
FBrf0226403-2.coIP.WB
Reference
He et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Met 

Met

bait

V5 tag

Hsp83 

Hsp83

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of Kc cell line; bait produced from transfected construct; prey produced from transfected construct.

(He et al., 2014)

Treatment of cells with methoprene, a juvenile hormone analog, increases interaction. Treatment of cells with geldanamycin, an Hsp90 inhibitor, abolishes interaction.

(He et al., 2014)
FBrf0253421-6.coIP.WB
Reference
Gao et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Kc
Participants
Corresponds to
Reported as
Role
Note
Met 

Met

bait

FLAG tag

Hsp83 

Hsp83

prey

V5 tag

Hsp83 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Hsp83 
phosphorylation residue 219
mutation decreasing interaction
aa 219

S219A; coordinates relative to Hsp83-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of Kc cell line; bait produced from transfected construct; prey produced from transfected construct.

(Gao et al., 2022)

Interaction increased in the presence of juvenile hormone analog.

(Gao et al., 2022)
FBrf0253421-7.coIP.WB
Reference
Gao et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Kc
Participants
Corresponds to
Reported as
Role
Note
Hsp83 

Hsp83

bait

V5 tag

Met 

Met

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction observed in the presence of juvenile hormone analog.

(Gao et al., 2022)

Source was cell extract of Kc cell line; bait produced from transfected construct; prey produced from transfected construct.

(Gao et al., 2022)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)