Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryonic extract.

eIF4E1 was coupled to m7-GTP (mRNA cap analog) sepharose and used to pull down proteins from embryonic extracts.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

eIF4E1 was coupled to m7-GTP (mRNA cap analog) sepharose and used to pull down in vitro translated bcd.