FB2026_03 , released September 17, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
trbd, Tak1
FlyBase ID
FBig0000109554
Interaction Network
Interactions Browser links
trbd network
Tak1 network
Reported Interactions
FBrf0224271-1.coIP.WB
Reference
Fernando et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
trbd 

trbd

bait

V5 tag

Tak1 

Tak1

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Fernando et al., 2014)
FBrf0254147-1.coIP.WB
Reference
Hua et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
trbd 

Trbd

bait

Myc tag

trbd 
Tak1 

Tak1

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
trbd 
NZF domain
necessary binding region
aa 1-261

coordinates relative to trbd-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Hua et al., 2022)

Treatment with heat killed E.coli or addition of PGRP-LC increased interaction, while knockdown of Rel reduced the interaction. This indicates a role for the IMD signaling pathway in the interaction between trbd and Tak1.

(Hua et al., 2022)
FBrf0254147-2.PD.WB
Reference
Hua et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
trbd 

Trbd

bait

GST tag

trbd 
Tak1 

Tak1

prey

His-Ub tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
trbd 
NZF domain
sufficient binding region
aa 1-261

coordinates relative to trbd-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait and prey produced a recombinant fusion proteins in bacterial system.

(Hua et al., 2022)
FBrf0254147-3.coIP.WB
Reference
Hua et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Tak1 

Tak1

bait

FLAG tag

Tak1 
trbd 

trbd

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Tak1 
All lysine residues
mutation decreasing interaction
unspecified

K-R mutation for all lysine residues.

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Hua et al., 2022)
FBrf0254147-4.coIP.WB
Reference
Hua et al., 2022
Description
physical association
Assay
Collection
Source/Stage
fat body / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Tak1 

Tak1

bait
trbd 

Trbd

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larval fat body lysate; bait produced from endogenous gene; prey produced from endogenous gene.

(Hua et al., 2022)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)