FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Rad23, Rpn10
FlyBase ID
FBig0000109591
Interaction Network
Interactions Browser links
Rad23 network
Rpn10 network
Reported Interactions
FBrf0217849-1.PD.WB
Reference
Lipinszki et al., 2012
Description
physical association
Assay
Collection
Source/Stage
organism / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rad23 

Rad23

bait

GST tag

Rad23 
Rpn10 

Rpn10

prey

Strep or FLAG tag

Rpn10 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Rpn10 
C-terminal domain
sufficient binding region
aa 206-396
Rad23 
N-terminal domain
sufficient binding region
aa 1-125

coordinates relative to Rad23-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transgenic larval extract.

(Lipinszki et al., 2012)
FBrf0217849-12.CH.WB
Reference
Lipinszki et al., 2012
Description
physical association
Assay
Collection
Source/Stage
organism / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rad23 

Rad23

bait

GST tag

Rad23 
Rpn10 

Rpn10

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Rad23 
N-terminal domain
sufficient binding region
aa 1-125

coordinates relative to Rad23-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larvae of transgenic fly line; bait produced as a recombinant fusion protein in bacterial system; prey produced from tagged transgenic construct.

(Lipinszki et al., 2012)

Rad23 largely bound to non-ubiquitylated, proteasome associated Rpn10.

(Lipinszki et al., 2012)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)