Pros54, p54, S5a
Gene model reviewed during 5.46
The 26S proteasome is composed of a core protease, known as the 20S proteasome, capped at one or both ends by the 19S regulatory complex (RC). The RC is composed of at least 18 different subunits in two subcomplexes, the base and the lid, which form the portions proximal and distal to the 20S proteolytic core, respectively (By similarity). Interacts with Ubc4.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Rpn10 using the Feature Mapper tool.
Expression levels of Rpn10 protein are high during embryonic, prepupal, and pupal stages. Expression levels are intermediate during late third instar (108-120 hours AEL), and at pharate and adult stages. Expression is low during first, second, and early third instar larval stages (36-108 hours AEL)
GBrowse - Visual display of RNA-Seq signalsView Dmel\Rpn10 in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: Rpn10 Pros54
The nomenclature of genes encoding subunits of the 26S proteasome of D. melanogaster have been standardized according to FBrf0215459. These symbols/names largely follow those used already in FlyBase, and largely mirror fly community usage. HOWEVER, note that at least one other nomenclature system exists that is followed by the HUGO Gene Nomenclature Committee (HGNC), for example, with the unfortunate result that several D. melanogaster genes have shared synonyms.
RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells.
Pros54 is a regulatory complex subunit of the 26S protease. The encoded protein is the multiubiquitin-chain-binding subunit. Assays of the recombinant protein reveals two multiubiquitin-binding domains in the subunit.
Pros54, a non-ATPase subunit of the regulatory complex of the 26S protease, has been cloned and sequenced.