FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Rpn10Pw+
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General Information
Symbol
Dmel\Rpn10Pw+
Species
D. melanogaster
Name
FlyBase ID
FBal0182142
Feature type
allele
Associated gene
Dmel\Rpn10
Associated Insertion(s)
Carried in Construct
Also Known As
Δp54
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
Delta2-3 transposase
(Szlanka et al., 2003)
Progenitor genotype
P{lacW}COX8S055418
(Szlanka et al., 2003)
Caused by aberration
Df(3L)pros54Pw+
Cytology
Description

In Pros54Pw+ more than 90% of the Pros54 coding region is deleted. The 5' regulatory region, the first exon and intron, and a short segment of the second exon are retained.

(Szlanka et al., 2003)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The Pros54Pw+ mutation causes polyphasic lethality during the first larval instar (40% mortality), second larval instar (20%), third larval instar (13%) and pupal (27%) stages.

      (Lipinszki et al., 2012)

      Pros54Pw+ mutants exhibit increased mortality compared to wild type in the first and second instar larval stage. The surviving larvae develop slowly, with the majority reaching maturity and pupariating 3-4 days later than wild-type larvae. Pros54Pw+ mutants, especially when late in pupariating, often have small internal organs compared to wild type. For example, the brain and ventral ganglion are about half the normal size. Only 27% of Pros54Pw+ mutants survive up to the end of larval development and form puparia. Such mutant puparia are smaller than the wild type and have a characteristic bent shape. The cuticle is softer, lighter in colour and less rigid than in the wild type. Some mutants only have pupal cuticles in the regions of the head and the external genitalia. About one-third of mutants secrete a complete pupal cuticle. However, the head eversion is mostly incomplete and the wings and legs are always smaller and shorter than in wild type. In half of Pros54Pw+ puparia only imaginal disc derivatives (head, thorax, adult appendages and the region of the external genitalia) secrete the pupal cuticle. All Pros54Pw+ puparia dry out in 1-2 days. The pupae never develop beyond this stage and even if kept wet to prevent drying out they are unable to develop further and die. Histolysis of the larval tissues apparently occurs and the fat body breaks up into single cells. Metamorphosis stops at this point because the inner organs of the adult are not formed and secretion of an adult cuticle never takes place. Neuroblast preparations from Pros54Pw+ larvae have a mitotic index of 4.1 compared to 1.7 in wild-type preparations. The metaphase to anaphase ratio is also higher in mutants (4.3 vs 2.2 in wild type). A significant proportion (24.6%) of Pros54Pw+ mitotic cells have over-condensed chromosomes which look similar to those seen in colchicine-induced mitotic arrest; mutants also have cells in prometaphase and metaphase that show no obvious centromeric connection between some of the sister chromatids. Additionally, some cells in anaphase display chromosome bridges and lagging chromosomes. In about 19% of Pros54Pw+ cells the major chromosomes are arranged in a circle with the centromeres pointing toward the centre and the fourth chromosomes in the middle of the circle, a pattern that may be caused by the formation of monopolar spindles. There is also a higher frequency of aneuploid and polyploid cells in Pros54Pw+ mutants (6% vs 0 in wild type). The presence of Pros54Pw+ in mutant pupae does not destabilise the regulatory complex of the 26S proteasome, nor does it interfere with the assembly of the regulatory complex and the catalytic core. Flies that are homozygous for Pros54Pw+ with one copy of Pros54t2.7 exhibit larval-pupal lethality. Flies that are homozygous for Pros54Pw+ with one copy of Pros54t2.8 are early larval lethals.

      (Szlanka et al., 2003)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressor of
      Statement
      Reference

      Rpn10Pw+ is a suppressor | partially of visible | adult stage phenotype of DebclUAS.Tag:HA, Scer\GAL4ptc.PU

      (Colin et al., 2014)

      Rpn10Pw+ is a suppressor of visible | adult stage phenotype of Mmus\BaxUAS.cGa, Scer\GAL4vg.PM

      (Colin et al., 2014)
      Phenotype Manifest In
      Suppressor of
      Statement
      Reference

      Rpn10Pw+ is a suppressor | partially of wing | adult stage phenotype of DebclUAS.Tag:HA, Scer\GAL4ptc.PU

      (Colin et al., 2014)

      Rpn10Pw+ is a suppressor of wing | adult stage phenotype of Mmus\BaxUAS.cGa, Scer\GAL4vg.PM

      (Colin et al., 2014)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Rpn10Pw+ partially suppresses the adult wing phenotype of flies expressing DebclScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4ptc.PU.

      (Colin et al., 2014)
      Xenogenetic Interactions
      Statement
      Reference

      Rpn10Pw+ partially suppresses the notched wing phenotype observed in flies expressing Mmus\BaxScer\UAS.cGa under the control of Scer\GAL4vg.PM.

      (Colin et al., 2014)
      Complementation and Rescue Data
      Rescued by

      Rpn10Pw+ is rescued by Scer\GAL4da.G32/Rpn10UAS.Tag:FLAG

      (Lipinszki et al., 2011)

      Rpn10Pw+ is rescued by Rpn10+t8

      (Szlanka et al., 2003)
      Partially rescued by

      Rpn10Pw+ is partially rescued by Rpn10ΔK.UAS.Tag:FLAG/Scer\GAL4da.G32

      (Lipinszki et al., 2012)
      Not rescued by

      Rpn10Pw+ is not rescued by Rpn10t2.8

      (Szlanka et al., 2003)

      Rpn10Pw+ is not rescued by Rpn10t2.7

      (Szlanka et al., 2003)
      Comments

      Expression of Pros54ΔK.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4da.G32 shifts the lethal phase of Pros54Pw+ mutants to the pharate adult stage.

      (Lipinszki et al., 2012)

      Expression of Pros54Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4da.G32 rescues the lethality of Pros54Pw+.

      (Lipinszki et al., 2011)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      Dp54
      (Colin et al., 2014)
      Pros54Pw+
      Rpn10Pw+
      Δp54
      (Colin et al., 2014, Lipinszki et al., 2012, Lipinszki et al., 2011)
      Name Synonyms
      Secondary FlyBase IDs
        References (4)