FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Ubqn, Rpn10
FlyBase ID
FBig0000096065
Interaction Network
Interactions Browser links
Ubqn network
Rpn10 network
Reported Interactions
FBrf0216858-1.PD
Reference
Lipinszki et al., 2011
Description
physical association
Assay
Collection
Source/Stage
organism / pupal stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Ubqn 

Dsk2

bait

GST pull down

Ubqn 
Rpn10 

p54

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Ubqn 
N-terminal region
sufficient binding region

\'NTH\'; N-terminal half

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was pupal extract.

(Lipinszki et al., 2011)
FBrf0216858-2.PD
Reference
Lipinszki et al., 2011
Description
physical association
Assay
Collection
Source/Stage
organism / pupal stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Ubqn 

Dsk2

bait

GST pull down

Ubqn 
Rpn10 

p54

prey

FLAG tag

Rpn10 
Rpn10 
Rpn10 
Rpn10 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Rpn10 
C-terminal region
sufficient binding region

\'p54-CTH\'; C-terminal half

Rpn10 
region
necessary binding region
aa 303-322

\'UIM3\'; ubiquitin-interacting motif 3

Rpn10 
A311T
necessary binding region
aa 311

\'UIM3(A311T)\'

Rpn10 
S315A
necessary binding region
aa 315

\'UIM3(S315A)\'

Ubqn 
N-terminal region
sufficient binding region

\'Dkl2-NTH\'; N-terminal half, includes UBL domain

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was pupal extract from transgenic line carrying tagged Pros54 construct.

(Lipinszki et al., 2011)
FBrf0217849-2.PD.WB
Reference
Lipinszki et al., 2012
Description
physical association
Assay
Collection
Source/Stage
organism / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Ubqn 

Ubqn

bait

GST tag

Ubqn 
Rpn10 

Rpn10

prey

Strep or FLAG tag

Rpn10 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Rpn10 
C-terminal domain
sufficient binding region
aa 206-396
Ubqn 
N-terminal domain
sufficient binding region
aa 1-252
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transgenic larval extract.

(Lipinszki et al., 2012)
FBrf0217849-7.CH.WB
Reference
Lipinszki et al., 2012
Description
physical association
Assay
Collection
Source/Stage
organism / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Ubqn 

Ubqn

bait

GST tag

Ubqn 
Rpn10 

Rpn10

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Ubqn 
N-terminal domain
sufficient binding region
aa 1-252
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larvae of transgenic fly line; bait produced as a recombinant fusion protein in bacterial system; prey produced from tagged transgenic construct.

(Lipinszki et al., 2012)

Ubqn largely bound to non-ubiquitylated, proteasome associated Rpn10.

(Lipinszki et al., 2012)
FBrf0231167-15.CS.WB
Reference
Kessler et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Ubqn 

Ubqn

neutral component
Ubqn 

Ubqn

neutral component
Ubqn 

Ubqn

neutral component
Rpn10 

Rpn10

neutral component
Rpn10 

Rpn10

neutral component
Rpn10 

Rpn10

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; proteins produced from endogenous genes.

(Kessler et al., 2015)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)