Source was cell extract of Kc cell line; bait produced and labeled by chemical synthesis; prey produced by endogenous gene.
Interaction in vitro; bait produced by in vitro transcription/translation; prey synthesized and labeled in vitro.
Interaction in vitro; bait synthesized and labeled in vitro; prey derived from wild-type or transgenic embryo extract.
Interaction in vitro; bait synthesized and labelled in vitro; prey derived from Kc cell nuclear extract or produced in baculovirus and insect cell system.
Interaction in vitro; bait synthesized and labelled in vitro; prey derived from S2 cell nuclear extract or produced in baculovirus and insect cell system.
125nt from His3 pre-mRNA. 2\'O-methyl modification to prevent cleavage and biotin tag provided by oligo annealed to 3-prime end of His3 pre-mRNA.
Interaction in vitro; bait produced by in vitro transcription; prey derived from Kc nuclear extract.
63nt from His3 pre-mRNA with 2\'O-methyl modification to prevent cleavage
Interaction in vitro; bait commercially synthesized; prey derived from Kc nuclear extract.
