FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
ena, Zyx
FlyBase ID
FBig0000109996
Interaction Network
Interactions Browser links
ena network
Zyx network
Reported Interactions
FBrf0157235-1.coIP.A
Reference
Renfranz et al., 2003
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Zyx 

Zyx102

bait

S[35] and cold antibody to Zyx

ena 

ena

prey

S[35]

ena 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ena 
interaction domain residue 97
mutation disrupting interaction
aa 97

A97V

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced and labeled by in vitro translation; prey produced and labeled by in vitro translation.

(Renfranz et al., 2003)

Both proteins were radiolabeled and incubated together and then they were precipitated with an antibody against Zyx.

(Renfranz et al., 2003)
FBrf0227860-1.coIP.WB
Reference
Gaspar et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
ena 

ena

bait

FLAG tag

Zyx 

Zyx

prey

HA tag

Zyx 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Zyx 
interaction domain
necessary binding region
aa 208-231

coordinates relative to Zyx-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Gaspar et al., 2015)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)