FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000110237
Interaction Network
Interactions Browser links
Arc2 network
Reported Interactions
FBrf0235788-913.Y2H
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Arc2 

Arc2-RA

bait

fused to GAL4 DNA-binding domain

Arc2 

Arc2-RA

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein.(Pairwise.)

MORATORIUM:This data was made available \'pre-publication\' and, as such, its use is subject to the specific limitations described in FBrf0235788 for the period of 12 months. Moratorium expires on June 13, 2018.

Two-hybrid system: yeast GAL4-BD/GAL4-AD

FBrf0235788-913.Y2Hpool
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Arc2 

Arc2-RA

bait

fused to GAL4 DNA-binding domain

Arc2 

Arc2-RA

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein.(Pooled)

MORATORIUM:This data was made available \'pre-publication\' and, as such, its use is subject to the specific limitations described in FBrf0235788 for the period of 12 months. Moratorium expires on June 13, 2018.

Two-hybrid system: yeast GAL4-BD/GAL4-AD

FBrf0244795-2.EM
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Arc2 

dArc2

neutral component
Arc2 
Arc2 

dArc2

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Arc2 
interaction domain
sufficient binding region
aa 2-193
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

Arc2 assembles into an icosahedral capsid.

FBrf0248964-1.XC
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Arc2 

dArc2

neutral component
Arc2 

dArc2

neutral component
Arc2 
Arc2 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Arc2 
N-lobe domain
sufficient binding region
aa 38-109

coordinates relative to Arc2-PA

Arc2 
C-lobe domain
sufficient binding region
aa 110-193

coordinates relative to Arc2-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Both the Arc2 N-lobe and C-lobe form dimers in crystallo.

Interaction in vitro; protein produced as a recombinant fusion protein in bacterial system.

FBrf0248964-3.SAXS
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Arc2 

dArc2

neutral component
Arc2 
Arc2 
Arc2 

dArc2

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Arc2 
N-lobe domain
sufficient binding region
aa 38-109

coordinates relative to Arc2-PA

Arc2 
C-lobe domain
sufficient binding region
aa 110-193

coordinates relative to Arc2-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; protein produced as a recombinant fusion protein in bacterial system.

The Arc2 C-lobe behaved as a dimer in solution while the N-lobe behaved as a tetramer.

FBrf0248964-5.CH.MW
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Arc2 

dArc2

neutral component
Arc2 

dArc2

neutral component
Arc2 
Arc2 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Arc2 
N-lobe domain
sufficient binding region
aa 38-109

coordinates relative to Arc2-PA

Arc2 
C-lobe domain
sufficient binding region
aa 110-193

coordinates relative to Arc2-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; protein produced as a recombinant fusion protein in bacterial system.

The Arc2 C-lobe behaved as a dimer in solution while the N-lobe behaved as a tetramer.

External Crossreferences and Linkouts ( 0 )
References (3)