FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
polo, nuf
FlyBase ID
FBig0000110267
Interaction Network
Interactions Browser links
polo network
nuf network
Reported Interactions
FBrf0235698-1.PD.WB
Reference
Brose et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
nuf 

nuf

bait

GST tag

polo 

polo

prey

MBP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in a baculovirus and insect cell system; prey produced as a recombinant fusion protein in a baculovirus and insect cell system.

(Brose et al., 2017)
FBrf0235698-2.ENZ.A
Reference
Description
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
polo 

polo

enzyme

His tag

polo 

polo

enzyme

His tag

nuf 

nuf

enzyme target

GST tag, P[32] label

nuf 

nuf

enzyme target

GST tag, P[32] label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)