FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
fwd, Rab11
FlyBase ID
FBig0000110514
Interaction Network
Interactions Browser links
fwd network
Rab11 network
Reported Interactions
FBrf0209515-1.Y2H
Reference
Polevoy et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
fwd 

Fwd

bait

fused to GAL4 DNA-binding domain

fwd 
Rab11 

Rab11

prey

fused to GAL4 activation domain

Rab11 
Rab11 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
fwd 
kinase domain
mutation decreasing interaction
1191

D1191A

Rab11 
GTP-bound mutant
mutation increasing interaction
aa 70

Q70L

Rab11 
GDP-bound mutant
mutation increasing interaction
aa 25

S25N

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Polevoy et al., 2009)

Both Rab11 mutants showed increased binding to wild type and mutant fwd, however the Q70L mutation of Rab11 had a stronger interaction than the S25N. Nca, Mlc-c, sqh, Rab5, and Rab7 did not interact with fwd in this assay.

(Polevoy et al., 2009)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Polevoy et al., 2009)
FBrf0209515-2.coIP.WB
Reference
Polevoy et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rab11 

Rab11

bait

FLAG tag

fwd 

Fwd

prey

HA tag

fwd 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
fwd 
kinase domain
mutation decreasing interaction
1191

D1191A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of COS-7 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Polevoy et al., 2009)
FBrf0209515-3.coIP.WB
Reference
Polevoy et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
fwd 

Fwd

bait

HA tag

fwd 
Rab11 

Rab11

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
fwd 
kinase domain
mutation decreasing interaction
1191

D1191A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of COS-7 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Polevoy et al., 2009)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)