FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Sec13, Mad
FlyBase ID
FBig0000111557
Interaction Network
Interactions Browser links
Sec13 network
Mad network
Reported Interactions
FBrf0211334-1.coIP.WB
Reference
Chen and Xu, 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Sec13 

Sec13

bait

FLAG or HA tag

Mad 

Smad1

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct or endogenous gene.

(Chen and Xu, 2010)

Sec13 preferentially interacts with phosphorylated Mad.

(Chen and Xu, 2010)
FBrf0211334-2.coIP.WB
Reference
Chen and Xu, 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Mad 

Smad1

bait

FLAG tag

Sec13 

Sec13

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Chen and Xu, 2010)
FBrf0211334-3.PD.WB
Reference
Chen and Xu, 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Sec13 

Sec13

bait

GST tag

Mad 

Smad1

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey immunopurified from S2 cell extract.

(Chen and Xu, 2010)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)