Source was frog oocytes; bait produced from injected RNA construct; prey peptide was synthesized in vitro.

Interaction inferred from inward currents across the plasma membrane in response to peptide application, measured by voltage clamp.

Source was CHO/G-16 cell line; bait produced from transfected construct; prey peptide was synthesized in vitro.

Interaction inferred by IP3-calcium activation in response to peptide application, measured using apoaequorin bioluminescent sensor.