Chro, His1 Physical Interaction report

General Information

Interaction Type

physical association

Interacting Genes

Chro, His1

FlyBase ID

FBig0000112655

Interaction Network

Interactions Browser links

Chro network
His1 network

Reported Interactions

FBrf0218885-1.FB.WB

Reference

Yao et al., 2012

Description

physical association

Assay

filter binding

western blot

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

His1

bait

MBP tag

Chro

prey

GST tag

Chro

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Chro

chromo domain

sufficient binding region

aa 219-277

Chro

chromo domain

necessary binding region

aa 220-346

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Yao et al., 2012)

FBrf0218885-2.PD.WB

Reference

Yao et al., 2012

Description

physical association

Assay

pull down

western blot

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Chro

bait

GST tag

Chro

His1

prey

His tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Chro

chromo domain

sufficient binding region

aa 219-277

Chro

N-terminal domain

necessary binding region

aa 1-328

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Yao et al., 2012)

FBrf0218885-3.PD.WB

Reference

Yao et al., 2012

Description

physical association

Assay

pull down

western blot

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

His1

bait

His tag

Chro

prey

GST tag

Chro

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Chro

chromo domain

sufficient binding region

aa 219-277

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Yao et al., 2012)

FBrf0218885-4.PD.WB

Reference

Yao et al., 2012

Description

physical association

Assay

pull down

western blot

Collection

Source/Stage

Cell line used

S2-unspecified

Participants

Corresponds to

Reported as

Role

Note

Chro

bait

GST tag

Chro

His1

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Chro

chromo domain

sufficient binding region

aa 219-277

Chro

N-terminal domain

necessary binding region

aa 1-328

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from S2 cell nuclear extract.

(Yao et al., 2012)

FBrf0218885-5.PD.WB

Reference

Yao et al., 2012

Description

physical association

Assay

pull down

western blot

Collection

Source/Stage

Cell line used

S2-unspecified

Participants

Corresponds to

Reported as

Role

Note

His1

bait

His tag

Chro

prey

Chro

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Chro

chromo domain

sufficient binding region

aa 219-277

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from S2 cell nuclear extract.

(Yao et al., 2012)

FBrf0218885-6.coIP.WB

Reference

Yao et al., 2012

Description

physical association

Assay

anti tag coimmunoprecipitation

western blot

Collection

Source/Stage

organism / adult stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Chro

bait

GFP tag

Chro

His1

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Chro

chromo domain

sufficient binding region

aa 219-277

Chro

N-terminal domain

necessary binding region

aa 1-328

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Yao et al., 2012)

External Crossreferences and Linkouts ( 0 )

References (1)

Report Sections

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