Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Three-hybrid system: yeast lexA-BD/B42-AD, bgcn was coexpressed as a bridging factor in a yeast three-hybrid assay with bam as bait and pum as prey.

Source was live HEK293 cells; proteins produced from transfected constructs.

bgcn was coexpressed as a bridging factor in a fluorescence complementation assay with bam as bait and pum as prey.