FB2025_01 , released February 20, 2025
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
edl, pnt
FlyBase ID
FBig0000112968
Interaction Network
Interactions Browser links
edl network
pnt network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0135901-3.PD.A
Reference
Baker et al., 2001
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pnt 

pnt-P2

bait

GST tag

edl 

Mae

prey

[35]S label

edl 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
edl 
interaction domain residue 139
mutation disrupting interaction
aa 139

G139P

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Baker et al., 2001)
FBrf0157239-2.coIP.WB
Reference
Tootle et al., 2003
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
edl 

MAE

bait

MYC tag

pnt 

PNT-P2

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Tootle et al., 2003)
FBrf0161068-1.PD.A
Reference
Yamada et al., 2003
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pnt 

PNTP2

bait

GST tag

pnt 
edl 

edl

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
pnt 
interaction domain
sufficient binding region
aa 1-276
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Yamada et al., 2003)
FBrf0190365-2.SPR
Reference
Qiao et al., 2006
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pnt 

Pnt-P2

bait

GST,His tag

pnt 
pnt 
edl 

Mae

prey

MBP,His tag

edl 
edl 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
pnt 
SAM domain
sufficient binding region
aa 142-249
edl 
SAM domain
sufficient binding region
aa 94-173
pnt 
SAM domain residue 238
mutation disrupting interaction
aa 238

I238R

edl 
SAM domain residue 141
mutation disrupting interaction
aa 141

A141D

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as recombinant fusion proteins in bacterial system.

(Qiao et al., 2006)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (4)