FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000113841
Interaction Network
Interactions Browser links
mir-312 network
ttk network
Reported Interactions
FBrf0187121-5a.miR-mRNA.LUC
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

mir-312

bait
ttk 

ttk

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was S2 cell line; bait produced from transfected construct; prey produced from transfected reporter construct.

luciferase reporter assay

FBrf0231243-11a.miR-mRNA.LUC
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

mir-312

bait
ttk 

Dttk

prey
ttk 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ttk 
3-prime UTR
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was S2 cell line; bait produced from transfected construct; prey produced from transfected reporter construct.

The related mir-310, mir-311, mir-312 and mir-313 miRNAs were overexpressed together.

luciferase reporter assay

FBrf0231243-23a.miR-mRNA.PCR
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

mir-312

bait
ttk 

Dttk

prey
ttk 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ttk 
3-prime UTR
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The Df(2R)KT40 deletion was used, which removes mir-310, mir-311, mir-312 and mir-313.

Source was adult females; bait produced from endogenous gene; prey produced from endogenous gene.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)