FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
CycB, Psc
FlyBase ID
FBig0000114014
Interaction Network
Interactions Browser links
CycB network
Psc network
Reported Interactions
FBrf0218404-29.coIP.WB
Reference
Mohd-Sarip et al., 2012
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Psc 

PSC

bait
CycB 

CYC-B

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Mohd-Sarip et al., 2012)
FBrf0218404-53.coIP.WB
Reference
Mohd-Sarip et al., 2012
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
CycB 

CYC-B

bait
Psc 

PSC

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Mohd-Sarip et al., 2012)
FBrf0218404-6.coIP.MS
Reference
Mohd-Sarip et al., 2012
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Psc 

PSC

bait
CycB 

CYC-B

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Mohd-Sarip et al., 2012)
FBrf0218404-73.PD.A
Reference
Mohd-Sarip et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
CycB 

CYC-B

bait

GST tag

Psc 

PSC

prey

[35]S label

Psc 
Psc 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Psc 
interaction domain
sufficient binding region
aa 1321-1601
Psc 
interaction domain
necessary binding region
aa 721-1601
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Mohd-Sarip et al., 2012)
FBrf0218404-75.PD.WB
Reference
Mohd-Sarip et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
CycB 

CYC-B

bait

GST tag

Psc 

PSC

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in baculovirus and insect cell system.

(Mohd-Sarip et al., 2012)
FBrf0218404-76.ENZ.WB
Reference
Description
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Psc 

PSC

enzyme

FLAG tag

Psc 

PSC

enzyme

FLAG tag

Psc 
CycB 

CYC-B

enzyme target

His tag

CycB 

CYC-B

enzyme target

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)