FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
Sumo, sens
FlyBase ID
FBig0000114398
Interaction Network
Interactions Browser links
Sumo network
sens network
Reported Interactions
FBrf0218761-2.Y2H
Reference
Powell et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Sumo 

SUMO

bait

fused to GAL4 DNA-binding domain

Sumo 
Sumo 
Sumo 
Sumo 
sens 

SENS

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Sumo 
N-terminal domain
sufficient binding region
aa 1-88

deletion of C-terminal 2 amino acids

Sumo 
C-terminal domain
mutation decreasing interaction
aa 85-90

deletion of C-terminal 6 amino acids

Sumo 
putative sumoylation site
mutation decreasing interaction
aa 509

K509R

Sumo 
putative sumoylation motif
mutation decreasing interaction
aa 511

D511A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Powell et al., 2012)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Powell et al., 2012)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)