FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
fray, Ncc69
FlyBase ID
FBig0000115220
Interaction Network
Interactions Browser links
fray network
Ncc69 network
Reported Interactions
FBrf0212638-1.Y2H
Reference
Leiserson et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
fray 

Fray

bait

fused to GAL4 DNA-binding domain

fray 
fray 
Ncc69 

Ncc69

prey

fused to GAL4 activation domain

Ncc69 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Ncc69 
N-terminal domain
sufficient binding region
aa 1-163

coordinates relative to Ncc69-PA

fray 
PF2 domain
sufficient binding region
unspecified
fray 
PF2 domain
necessary binding region
unspecified
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Leiserson et al., 2011)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Leiserson et al., 2011)
FBrf0238827-2.ENZ.A
Reference
Description
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
fray 

Fray

enzyme

GST tag

fray 

Fray

enzyme

GST tag

fray 
Ncc69 

NKCC

enzyme target

GST tag, P[32] label

Ncc69 

NKCC

enzyme target

GST tag, P[32] label

Ncc69 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)