Source was nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

The Dek-EcR interaction was detected with or without the synthetic ecdysone, Muristerone A, indicating it is ligand independent.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in baculovirus and insect cell system.

usp was also added to the pulldown.